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bkdAA [2019-07-04 14:29:06]
2-oxoisovalerate dehydrogenase (E1 alpha subunit)
function
utilization of branched-chain keto acids
product
2-oxoisovalerate dehydrogenase (E1 alpha subunit)
synonyms
bfmBAA, bfmB1a, bkd
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.9|Utilization of branched-chain amino acids]Gene
Coordinates
2,499,090 2,500,082
The protein
Catalyzed reaction/ biological activity
3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO2 (according to Swiss-Prot)Protein family
BCKDHA family (according to Swiss-Prot)Paralogous protein(s)
[protein|B0A333BDFAE816B48B856A1DD91B73C41584A8E4|AcoA], [protein|953DE0F0B81894ECFF4C0693511AC238BF3D0C0A|PdhA] Structure
[PDB|1UMB] (from '' Thermus thermophilus'', 42% identity, 57% similarity) [Pubmed|15033367][SW|Localization]
Membrane-proximal (Spotty) [Pubmed|16479537]Expression and Regulation
Operons
genes
[gene|1CFF7202AC6078B6BAD1253F77FD4FE227C046DB|ptb]-[gene|A52E50104B18D0A8518218C52D9CC36FDDB29AAF|bcd]-[gene|8E08A0333E4EC89003DA3C1DD8DCD39CDDC8624C|buk]-[gene|1FD06CB6E81C920EFC656DBE2A13D68B1AA68872|lpdV]-[gene|9F298088C0A9EB7FE140C935AFC9243C6D4DE8AE|bkdAA]-[gene|A024921961A786294199EA12E04456C890ED8D8C|bkdAB]-[gene|262C9FD20C7A70B6F1FEB57735FA800F38EAB25A|bkdB]
description
[Pubmed|12823818]
sigma factors
[protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|SigL]: sigma factor, [Pubmed|10094682], in [regulon|1118A21CC581B0470EC6E7C178F2523CDCA70F93|SigL regulon]regulatory mechanism
[protein|4CEBD81F485DD0B660E297FFC34A0F5270652184|BkdR]: activation, [Pubmed|10094682], in [regulon|4CEBD81F485DD0B660E297FFC34A0F5270652184|BkdR regulon][protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|10094682], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]regulation
induced in the presence of isoleucine or valine ([protein|search|BkdR]) [Pubmed|10094682]view in new tabBiological materials
Mutant
BKE24050 ([gene|9F298088C0A9EB7FE140C935AFC9243C6D4DE8AE|bkdAA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAAGCCCCTCCTTTAT, downstream forward: _UP4_TATGCGAAGTAGGGAGGAAGBKK24050 ([gene|9F298088C0A9EB7FE140C935AFC9243C6D4DE8AE|bkdAA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAAGCCCCTCCTTTAT, downstream forward: _UP4_TATGCGAAGTAGGGAGGAAGReferences
12427936,15241682,10094682,12823818,16479537,1886522